Beilstein J. Org. Chem.2014,10, 2293–2306, doi:10.3762/bjoc.10.239
demonstrate that the presented cross-linked DNA with an ethylene-linked A/T base pair analog at the target position is a useful tool to determine the base-flipping equilibrium constant of a base-flipping enzyme which lies mostly on the extrahelical side for M.TaqI.
Keywords: DNAbaseflipping; DNA cross-link
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Graphical Abstract
Figure 1:
Covalent cross-linking of a I6S/U4S or G6S/U4S base pair within duplex ODN 1∙2 (a) by bis-alkylatio...